/* sam_internal.h -- internal functions; not part of the public API.

Copyright (C) 2019-2020 Genome Research Ltd.

Permission is hereby granted, free of charge, to any person obtaining a copy

of this software and associated documentation files (the "Software"), to deal

in the Software without restriction, including without limitation the rights

to use, copy, modify, merge, publish, distribute, sublicense, and/or sell

copies of the Software, and to permit persons to whom the Software is

furnished to do so, subject to the following conditions:

The above copyright notice and this permission notice shall be included in

all copies or substantial portions of the Software.

THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR

IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,

FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL

THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER

LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING

FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER

DEALINGS IN THE SOFTWARE. */

#ifndef HTSLIB_SAM_INTERNAL_H

#define HTSLIB_SAM_INTERNAL_H

#include <errno.h>

#include <stdint.h>

#include "htslib/hts_defs.h"

#include "htslib/sam.h"

#ifdef __cplusplus

extern "C" {

#endif

// Used internally in the SAM format multi-threading.

int sam_state_destroy(samFile *fp);

int sam_set_thread_pool(htsFile *fp, htsThreadPool *p);

int sam_set_threads(htsFile *fp, int nthreads);

// Fastq state

int fastq_state_set(samFile *fp, enum hts_fmt_option opt, ...);

void fastq_state_destroy(samFile *fp);

// bam1_t data (re)allocation

int sam_realloc_bam_data(bam1_t *b, size_t desired);

static inline int realloc_bam_data(bam1_t *b, size_t desired)

{

if (desired <= b->m_data) return 0;

return sam_realloc_bam_data(b, desired);

}

static inline int possibly_expand_bam_data(bam1_t *b, size_t bytes) {

size_t new_len = (size_t) b->l_data + bytes;

if (new_len > INT32_MAX || new_len < bytes) { // Too big or overflow

errno = ENOMEM;

return -1;

}

if (new_len <= b->m_data) return 0;

return sam_realloc_bam_data(b, new_len);

}

/*

* Convert a nibble encoded BAM sequence to a string of bases.

*

* We do this 2 bp at a time for speed. Equiv to:

*

* for (i = 0; i < len; i++)

* seq[i] = seq_nt16_str[bam_seqi(nib, i)];

*/

static inline void nibble2base_default(uint8_t *nib, char *seq, int len) {

static const char code2base[512] =

"===A=C=M=G=R=S=V=T=W=Y=H=K=D=B=N"

"A=AAACAMAGARASAVATAWAYAHAKADABAN"

"C=CACCCMCGCRCSCVCTCWCYCHCKCDCBCN"

"M=MAMCMMMGMRMSMVMTMWMYMHMKMDMBMN"

"G=GAGCGMGGGRGSGVGTGWGYGHGKGDGBGN"

"R=RARCRMRGRRRSRVRTRWRYRHRKRDRBRN"

"S=SASCSMSGSRSSSVSTSWSYSHSKSDSBSN"

"V=VAVCVMVGVRVSVVVTVWVYVHVKVDVBVN"

"T=TATCTMTGTRTSTVTTTWTYTHTKTDTBTN"

"W=WAWCWMWGWRWSWVWTWWWYWHWKWDWBWN"

"Y=YAYCYMYGYRYSYVYTYWYYYHYKYDYBYN"

"H=HAHCHMHGHRHSHVHTHWHYHHHKHDHBHN"

"K=KAKCKMKGKRKSKVKTKWKYKHKKKDKBKN"

"D=DADCDMDGDRDSDVDTDWDYDHDKDDDBDN"

"B=BABCBMBGBRBSBVBTBWBYBHBKBDBBBN"

"N=NANCNMNGNRNSNVNTNWNYNHNKNDNBNN";

int i, len2 = len/2;

seq[0] = 0;

for (i = 0; i < len2; i++)

// Note size_t cast helps gcc optimiser.

memcpy(&seq[i*2], &code2base[(size_t)nib[i]*2], 2);

if ((i *= 2) < len)

seq[i] = seq_nt16_str[bam_seqi(nib, i)];

}

#if HTS_BUILD_IS_X86_64 \

&& HTS_COMPILER_HAS_TARGET_AND_BUILTIN_CPU_SUPPORTS \

&& HAVE_BUILTIN_CPU_SUPPORT_SSSE3

#include "immintrin.h"

/*

* Convert a nibble encoded BAM sequence to a string of bases.

*

* Using SSSE3 instructions, 16 codepoints that hold 2 bases each can be

* unpacked into 32 indexes from 0-15. Using the pshufb instruction these can

* be converted to the IUPAC characters.

* It falls back on the nibble2base_default function for the remainder.

*/

__attribute__((target("ssse3")))

static inline void nibble2base_ssse3(uint8_t *nib, char *seq, int len) {

seq[0] = 0;

const char *seq_end_ptr = seq + len;

char *seq_cursor = seq;

uint8_t *nibble_cursor = nib;

const char *seq_vec_end_ptr = seq_end_ptr - (2 * sizeof(__m128i));

__m128i first_upper_shuffle = _mm_setr_epi8(

0, -1, 1, -1, 2, -1, 3, -1, 4, -1, 5, -1, 6, -1, 7, -1);

__m128i first_lower_shuffle = _mm_setr_epi8(

-1, 0, -1, 1, -1, 2, -1, 3, -1, 4, -1, 5, -1, 6, -1, 7);

__m128i second_upper_shuffle = _mm_setr_epi8(

8, -1, 9, -1, 10, -1, 11, -1, 12, -1, 13, -1, 14, -1, 15, -1);

__m128i second_lower_shuffle = _mm_setr_epi8(

-1, 8, -1, 9, -1, 10, -1, 11, -1, 12, -1, 13, -1, 14, -1, 15);

__m128i nuc_lookup_vec = _mm_lddqu_si128((__m128i *)seq_nt16_str);

/* Work on 16 encoded characters at the time resulting in 32 decoded characters

Examples are given for 8 encoded characters A until H to keep it readable.

Encoded stored as |AB|CD|EF|GH|

Shuffle into |AB|00|CD|00|EF|00|GH|00| and

|00|AB|00|CD|00|EF|00|GH|

Shift upper to the right resulting into

|0A|B0|0C|D0|0E|F0|0G|H0| and

|00|AB|00|CD|00|EF|00|GH|

Merge with or resulting into (X stands for garbage)

|0A|XB|0C|XD|0E|XF|0G|XH|

Bitwise and with 0b1111 leads to:

|0A|0B|0C|0D|0E|0F|0G|0H|

We can use the resulting 4-bit integers as indexes for the shuffle of

the nucleotide lookup. */

while (seq_cursor < seq_vec_end_ptr) {

__m128i encoded = _mm_lddqu_si128((__m128i *)nibble_cursor);

__m128i first_upper = _mm_shuffle_epi8(encoded, first_upper_shuffle);

__m128i first_lower = _mm_shuffle_epi8(encoded, first_lower_shuffle);

__m128i shifted_first_upper = _mm_srli_epi64(first_upper, 4);

__m128i first_merged = _mm_or_si128(shifted_first_upper, first_lower);

__m128i first_indexes = _mm_and_si128(first_merged, _mm_set1_epi8(15));

__m128i first_nucleotides = _mm_shuffle_epi8(nuc_lookup_vec, first_indexes);

_mm_storeu_si128((__m128i *)seq_cursor, first_nucleotides);

__m128i second_upper = _mm_shuffle_epi8(encoded, second_upper_shuffle);

__m128i second_lower = _mm_shuffle_epi8(encoded, second_lower_shuffle);

__m128i shifted_second_upper = _mm_srli_epi64(second_upper, 4);

__m128i second_merged = _mm_or_si128(shifted_second_upper, second_lower);

__m128i second_indexes = _mm_and_si128(second_merged, _mm_set1_epi8(15));

__m128i second_nucleotides = _mm_shuffle_epi8(nuc_lookup_vec, second_indexes);

_mm_storeu_si128((__m128i *)(seq_cursor + 16), second_nucleotides);

nibble_cursor += sizeof(__m128i);

seq_cursor += 2 * sizeof(__m128i);

}

nibble2base_default(nibble_cursor, seq_cursor, seq_end_ptr - seq_cursor);

}

static void (*nibble2base)(uint8_t *nib, char *seq, int len) = nibble2base_default;

__attribute__((constructor))

static void nibble2base_resolve(void) {

if (__builtin_cpu_supports("ssse3")) {

nibble2base = nibble2base_ssse3;

}

}

#else

static inline void nibble2base(uint8_t *nib, char *seq, int len) {

nibble2base_default(nib, seq, len);

}

#endif

#ifdef __cplusplus

}

#endif

#endif